NLM DIR Seminar Schedule
UPCOMING SEMINARS
RECENT SEMINARS
-
Dec. 17, 2024 Joey Thole
Training set associations drive AlphaFold initial predictions of fold-switching proteins -
Dec. 10, 2024 Amr Elsawy
AI for Age-Related Macular Degeneration on Optical Coherence Tomography -
Dec. 3, 2024 Sarvesh Soni
Toward Relieving Clinician Burden by Automatically Generating Progress Notes -
Nov. 19, 2024 Benjamin Lee
Reiterative Translation in Stop-Free Circular RNAs -
Nov. 12, 2024 Devlina Chakravarty
Fold-switching reveals blind spots in AlphaFold predictions
Scheduled Seminars on May 5, 2022
Contact NLMDIRSeminarScheduling@mail.nih.gov with questions about this seminar.
Abstract:
Insertion sequences of IS200/IS605 and IS607 usually contain only the genes that are required for their transposition and its regulation. These elements encode tnpA transposase, which is essential for mobilization, and often carry an accessory tnpB gene. Previous studies showed that TnpB might be a predecessor of the CRISPR–Cas9/Cas12 nucleases, and recently shown to be a RNA-directed nuclease that is guided by an RNA. In our work we provide a pipeline for systematic identification and characterization for TnpB genes. We obtain information of TnpB mobility obtained from completely sequenced genomes. We use phylogeny based methods to identify novel conserved TnpB families using their mobility and propose their novel function. This study expands our understanding of TnpB diversity and its applicability in genome editing.